| 2025-01-18 14:30:0018/01/2025 | FC ‘s-Gravenzande — UNA | 1 - 1 | N/B |
| 2025-01-18 14:30:0018/01/2025 | FC Lisse — Kozakken Boys | 1 - 0 | N/B |
| 2025-01-18 14:30:0018/01/2025 | Rijnvogels — Kloetinge | 3 - 6 | N/B |
| 2025-01-18 14:30:0018/01/2025 | Hoek — SteDoCo | 1 - 1 | N/B |
| 2025-01-18 15:00:0018/01/2025 | Blauw Geel’38 — VVSB | 4 - 0 | N/B |
| 2025-01-18 15:30:0018/01/2025 | OJC Rosmalen — Smitshoek | 3 - 2 | N/B |
| 2025-01-18 18:00:0018/01/2025 | Gemert — ASWH | 0 - 3 | N/B |
| 2025-01-18 19:30:0018/01/2025 | GSV’28 — SV Laar | 1 - 1 | N/B |
| 2025-01-18 19:30:0018/01/2025 | RVU — Maastricht West | 1 - 0 | N/B |
| 2025-01-19 13:30:5719/01/2025 | ZSV — RKSV Heeze. | 3 - 1 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Quick — TOGB | 3 - 3 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Dongen — Nuenen | 0 - 1 | N/B |
| 2025-01-19 14:00:0019/01/2025 | UDI’19 — Orion | 2 - 1 | N/B |
| 2025-01-19 14:00:0019/01/2025 | EVV — RBC | 1 - 3 | N/B |
| 2025-01-19 14:00:0019/01/2025 | SV Venray — Wittenhorst | 2 - 0 | N/B |
| 2025-01-19 14:00:0019/01/2025 | AWC — MASV | 1 - 1 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Juliana’31 — Mierlo Hout | 4 - 2 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Sparta’25 — De Valk | 3 - 1 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Bruheze — Rhode | 1 - 0 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Avanti’31 — NWC | 3 - 1 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Handel — RPC | 1 - 1 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Beerse Boys — RKVVO | 1 - 3 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Hoogeloon — Bergeijk | 1 - 3 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Terlo — FC Tilburg | 2 - 3 | N/B |
| 2025-01-19 14:00:0019/01/2025 | De Weebosch — Casteren | 0 - 3 | N/B |
| 2025-01-19 14:00:0019/01/2025 | TSC — Gilze | 2 - 0 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Bladella — Reusel Sport | 1 - 0 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Cluzona — SVSSS | 2 - 1 | N/B |
| 2025-01-19 14:00:0019/01/2025 | Mariahout — Sparta'18 | 1 - 2 | N/B |
| 2025-01-19 14:00:2919/01/2025 | HMVV — Rood Wit V. | 1 - 2 | N/B |
| 2025-01-19 14:00:3319/01/2025 | EMK — Sterksel | 2 - 1 | N/B |
| 2025-01-19 14:30:0019/01/2025 | SV Meerssen — GOES | 3 - 2 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Best Vooruit — Groene Ster | 0 - 4 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Valkenswaard — Baronie | 1 - 1 | N/B |
| 2025-01-19 14:30:0019/01/2025 | SC Susteren — Deurne | 3 - 1 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Bekkerveld — BSV Limburgia | 0 - 2 | N/B |
| 2025-01-19 14:30:0019/01/2025 | De Ster — Boekel Sport | 1 - 0 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Stiphout Vooruit — SBC | 0 - 2 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Oirschot Vooruit — Erp | 1 - 1 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Schaesberg — Veritas | 4 - 3 | N/B |
| 2025-01-19 14:30:0019/01/2025 | SHH — Merefeldia | 3 - 0 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Caesar — Geusselt Sport | 2 - 0 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Sp. Heerlen — ALFA SPORT | 0 - 4 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Unitas’59 — Spoordonkse Boys | 4 - 0 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Gestel — Acht | 1 - 4 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Wilhelmina Boys — DBS | 3 - 2 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Dommelen — FC Eindhoven/av | 4 - 1 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Brabantia — PSV/av | 0 - 1 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Moerse Boys — JEKA | 0 - 3 | N/B |
| 2025-01-19 14:30:0019/01/2025 | GSBW — Roosendaal | 3 - 2 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Rijen — VOAB | 2 - 0 | N/B |
| 2025-01-19 14:30:0019/01/2025 | Gastel — Uno Animo | 4 - 0 | N/B |
| 2025-01-19 14:30:0319/01/2025 | Milheezer B — Nieuw Woensel | 2 - 1 | N/B |
| 2025-01-21 20:00:0021/01/2025 | UNA — OJC Rosmalen | 20:00:0020:00 | N/B |
| 2025-01-24 19:30:0024/01/2025 | Kloetinge — GOES | 19:30:0019:30 | N/B |